Susceptibility of stored-product psocids to aerosol insecticides

The efficacies of commercial methoprene and esfenvalerate aerosols for control of stored-product psocid pests were evaluated in simulated field studies. The efficacies of methoprene, esfenvalerate EC, the carrier Isopar-M™, and a combination of methoprene and esfenvalerate aerosols for control of Liposcelis decolor (Pearman) (Psocoptera: Liposcelididae) and Liposcelis entomophila (Enderlein) nymphs were assessed, and the effects of direct and indirect exposure of Liposcelis bostrychophila Badonnel, L. decolor, and Liposcelis paeta Pearman adults to esfenvalerate EC aerosol were evaluated. The greatest nymphal mortality attained was 76%, indicating that the four aerosols tested were ineffective against L. decolor and L. entomophila nymphs. In the direct and indirect exposure studies, the greatest adult mortalities attained for the three psocid species were 62 and 32%, respectively. Based on these data, esfenvalerate aerosol is ineffective for control of L. bostrychophila, L. decolor, L. entomophila, and L. paeta psocid species. This study shows that methoprene, esfenvalerate EC, and a combination of methoprene and esfenvalerate aerosols were ineffective against the four psocid species tested when applied at rates that are usually effective against other stored-product insect pests

Insecticidal Effect of Diatomaceous Earth Against Three Species of Stored-Product Psocids on Maize, Rice, and Wheat

We evaluated the efficacy of three diatomaceous earth (DE) formulations, Dryacide, Protect-It, and Insecto, against three Psocoptera species, Liposcelis entomophila (Enderlein) (Liposcelididae), Lepinotus reticulatus Enderlein (Trogiidae), and Liposcelis decolor (Pearman), in the laboratory. Bioassays were conducted in three grain commodities, wheat, rice and maize, at 30°C and 75% RH, and the DEs were applied at the recommended dose rates of 1,000, 400, and 500 ppm for Dryacide, Protect-It, and Insecto, respectively. Differences in adult mortality were found among grains and DEs for L. entomophila and L. reticulatus, but these trends were not consistent for all combinations tested. Type of grain and DE did not affect L. decolor mortality significantly. Moreover, mortality increased with increasing exposure time for L. entomophila and L. reticulatus, but there was no effect of exposure time on L. decolor. After 7 d of exposure, mortalities of L. entomophila, L. reticulatus, and L. decolor were 56, 55, and 40%, respectively, and the respective mortality levels after 14 d were 63, 71, and 42%. Progeny production after 30 d was significantly suppressed for all species in the treated grains. However, progeny production was still high in the treated grains and reached 54, 42, and 76 individuals/10 g of grain for L. entomophila, L. reticulatus, and L. decolor, respectively. Progeny production did not vary with commodity. Our results suggest that DEs, when used alone, will not provide effective control of psocids

Drivers of farmers’ adoption of hermetic storage bags in Ghana

Post-harvest loss is a global challenge due to its serious threat to food security. Hermetic storage bags offer one way to combat the loss of food after harvest. The rate at which farmers adopt hermetic bags largely depends upon their access to information and training about the technology. The adoption of hermetic storage bags in Dormaa, Ghana, was the focus of this quantitative cross-sectional research study. This study sought to describe maize farmers’ perceptions of the hermetic storage bags in Dormaa, Ghana, based on the perceived innovation attributes and to ascertain farmers’ stages of adoption of the hermetic storage bags using Rogers’s (2003) innovation-decision model. A multistage systematic sampling technique was used to survey 217 maize farmers in four communities where maize production was the main economic activity. Data indicated that the largest group of farmers were at the confirmation stage of Rogers’ model. The logistic regression model was used to ascertain the influence of the innovation attributes on adoption. The findings reveal that of the five innovation characteristics, relative advantage, compatibility, and complexity are the key and significant drivers of the adoption of hermetic storage bags, with relative advantage and compatibility increasing the adoption likelihood and complexity reducing it. The study, therefore, recommends that communities lagging behind others in adoption should be targeted for additional training

Evaluation of plastic and steel bins for protection of stored maize against Insect Infestation in Ghana: Poster

Maize is a staple food in Ghana where there is ever increasing demand for its use to also support poultry and livestock production. However, post-harvest loss of maize is high in Ghana. This study evaluated the effectiveness of plastic and steel bins as bulk storage structures to reduce maize post-harvest loss in Ejura, Ghana during the period from February 2016 to January 2017. Maize pre-disinfested with a solar biomass hybrid dryer was stored in the following treatments: i. a white 7-ton plastic bin filled with untreated maize, ii. agreen 7-ton plastic bin filled with untreated maize, iii.a 6-ton Kikapu steel bin filled with untreated maize, iv. six 50-kg polypropylene (PP) bags filled with maize treated with Betallic Super (80 g pirimiphos-methyl and 15 g permethrin per liter as an emulsifiable concentrate (EC)), and v. six 50-kg PP bags filled with untreated maize ascontrol. Moisture content, insect pests, insect damaged kernels (IDK), grain weight loss, aflatoxin and fumonisin levels data were collected monthly. Sitophilus zeamais, Tribolium castaneum, Cathartus quadricollis, and Cryptolestes ferrugineus were the dominant insect species collected from maize samples. At the end of 12 months of storage, % IDK in the control was >17% while IDK values in the other treatments were <3%. Mean grain weight losses of <1% were recorded in the bin treatments. Mycotoxin levels in the control were above the allowable threshold of 15 ppb. Our data suggest that use of plastic and steel bins has potential to reduce post-harvest loss of maize during storage.Maize is a staple food in Ghana where there is ever increasing demand for its use to also support poultry and livestock production. However, post-harvest loss of maize is high in Ghana. This study evaluated the effectiveness of plastic and steel bins as bulk storage structures to reduce maize post-harvest loss in Ejura, Ghana during the period from February 2016 to January 2017. Maize pre-disinfested with a solar biomass hybrid dryer was stored in the following treatments: i. a white 7-ton plastic bin filled with untreated maize, ii. agreen 7-ton plastic bin filled with untreated maize, iii.a 6-ton Kikapu steel bin filled with untreated maize, iv. six 50-kg polypropylene (PP) bags filled with maize treated with Betallic Super (80 g pirimiphos-methyl and 15 g permethrin per liter as an emulsifiable concentrate (EC)), and v. six 50-kg PP bags filled with untreated maize ascontrol. Moisture content, insect pests, insect damaged kernels (IDK), grain weight loss, aflatoxin and fumonisin levels data were collected monthly. Sitophilus zeamais, Tribolium castaneum, Cathartus quadricollis, and Cryptolestes ferrugineus were the dominant insect species collected from maize samples. At the end of 12 months of storage, % IDK in the control was >17% while IDK values in the other treatments were <3%. Mean grain weight losses of <1% were recorded in the bin treatments. Mycotoxin levels in the control were above the allowable threshold of 15 ppb. Our data suggest that use of plastic and steel bins has potential to reduce post-harvest loss of maize during storage

Maize farmers’ use, preference, and trustworthiness of information sources and communication channels in Dormaa, Ghana: A gender comparison.

The study aimed to identify maize farmers\u27 use, preference, and trustworthiness of the various information sources and communication channels farmers in Dormaa, Ghana, used to receive information about their farming activities. A questionnaire was developed, and data was collected from 217 maize farmers. The result revealed more males are involved in maize production than females. Most males indicated they received training in using hermetic bags more than female farmers. Respondents ranked Extension agents highest as the source from which they receive their farming information. Based on the level of preference for the information sources, the results show a statistically significant difference between male and female farmers\u27 preference for Extension agents, friends/neighbors, and church leaders, with male farmers preferring these information sources more than females. The result indicated a significant difference between male and female preferences for phone calls, with more males indicating they preferred telephone calls. The t-test results of farmers’ trustworthiness for the information sources revealed a significant difference between male and female farmers’ trustworthiness for fellow farmers, friends/neighbors, telephone calls, and posters/billboards for communication channels. Given that Extension professionals are the most preferred and trustworthy source of information, we recommend that the government and stakeholders organize extension training programs to strengthen local extension. We recommend greater women involvement and given strategic roles in the planning and organization of training programs

Mitochondrial genome organization varies among different groups of the booklouse, Liposcelis bostrychophila: Poster

The booklouse, Liposcelis bostrychophila is an important stored pest worldwide. The mt genome of an asexual strain (Beibei, China) of the booklouse, L. bostrychophila, comprises two chromosomes; each chromosome contains approximate half of the 37 genes typically found in animals. The mt genomes of two sexual strains of L. bostrychophila, however, comprise five and seven chromosomes respectively; each chromosome contains one to six genes. To understand mt genome evolution in L. bostrychophila, we sequenced the mt genomes of six strains of asexual L. bostrychophila collected from different locations in China, Croatia and USA. The mt genomes of all of the six asexual strains of L. bostrychophila collected in China, Croatia and USA have two chromosomes. Phylogenetic analysis of mt genome sequences divided nine strains of L. bostrychophila into four groups. Each group has a distinct mt genome organization and substantial sequence divergence (48.7-87.4%) from other groups. Furthermore, the seven asexual strains of L. bostrychophila including the published Beibei strain are more closely related to two other species of booklice, L. paeta and L. sculptilis, than to the sexual strains of L. bostrychophila. Our results revealed highly divergent mt genomes in the booklouse, L. bostrychophila, and indicated that L. bostrychophila is a cryptic species.The booklouse, Liposcelis bostrychophila is an important stored pest worldwide. The mt genome of an asexual strain (Beibei, China) of the booklouse, L. bostrychophila, comprises two chromosomes; each chromosome contains approximate half of the 37 genes typically found in animals. The mt genomes of two sexual strains of L. bostrychophila, however, comprise five and seven chromosomes respectively; each chromosome contains one to six genes. To understand mt genome evolution in L. bostrychophila, we sequenced the mt genomes of six strains of asexual L. bostrychophila collected from different locations in China, Croatia and USA. The mt genomes of all of the six asexual strains of L. bostrychophila collected in China, Croatia and USA have two chromosomes. Phylogenetic analysis of mt genome sequences divided nine strains of L. bostrychophila into four groups. Each group has a distinct mt genome organization and substantial sequence divergence (48.7-87.4%) from other groups. Furthermore, the seven asexual strains of L. bostrychophila including the published Beibei strain are more closely related to two other species of booklice, L. paeta and L. sculptilis, than to the sexual strains of L. bostrychophila. Our results revealed highly divergent mt genomes in the booklouse, L. bostrychophila, and indicated that L. bostrychophila is a cryptic species

Acute lethal and behavioral sublethal responses of two stored-product psocids to surface insecticides

BACKGROUND: The psocids Liposcelis bostrychophila Badonnel and L. entomophila (Enderlein) (Liposcelididae) are emerging pests of stored products. Although their behavior, particularly their high mobility, may contribute to the reported relatively low efficacy of insecticides against them, studies to investigate this have not been conducted. The present study aimed to assess the label rate efficacy of three commercial insecticides (β-cyfluthrin, chlorfenapyr and pyrethrins) applied on concrete surfaces against L. bostrychophila and L. entomophila, and also their sublethal effect on the mobility of these species. RESULTS: The synthetic insecticides β-cyfluthrin and chlorfenapyr showed high short-term efficacy (LT95 ≤ 15 h) against both psocid species, unlike the natural pyrethrins (LT95 ≥ 4 days). Liposcelis bostrychophila was slightly more tolerant (≥1.2×) than L. entomophila to all three insecticides. Behavioral assays with fully sprayed and half-sprayed concrete arenas indicated that the insecticides reduced the mobility of both species. Pyrethrins seem to elicit weak repellence in L. bostrychophila. CONCLUSION: β-Cyfluthrin and chlorfenapyr were effective against both psocid species, but not pyrethrins. The mobility of both species does not seem to play a major role in the differential selectivity observed, but the lower mobility of L. bostrychophila may be a contributing factor to its higher insecticide tolerance

Effectiveness of the Zerofly storage bag fabric against stored-product insects

The ZeroFly Storage Bag is a woven polypropylene bag (PP) that has deltamethrin incorporated in its fibers, and represents a novel approach to reducing stored-product insect pest-related postharvest losses. Fabric samples from ZeroFly bags, polypropylene (PP) bags, jute bags, malathion-treated PP bags, malathion-treated jute bags and GrainPro bags were affixed to the bottom of 9-cm Petri dishes and 20 adults of either Sitophilus oryzae (L.) or Tribolium castaneum (Herbst) were introduced to determine contact sensitivity of insects exposed to ZeroFly bag fabric. Knockdown, mortality and number of progeny were recorded for different exposure periods (24, 48 or 72 h) and oviposition periods (7, 14 or 21 d). Additionally, mini bags were made from ZeroFly bags, PP bags, laminated PP bags and jute bags, and used to determine ability of adult S. oryzae, T. castaneum and Rhyzopertha dominica (F.) to chew through the bags and efficacy of ZeroFly bags at preventing insect infestations from outside and to contain infestations within bags. Knockdown assessment for ZeroFly bag fabric showed that time required to knockdown 99% of S. oryzae and T. castaneum was <3 h. For 72-h exposure to ZeroFly bag fabric, mortalities for S. oryzae and T. castaneum were 76.7 and 62.2%, respectively; mortality was </=6% in other fabrics. ZeroFly bag fabric also significantly suppressed progeny production by S. oryzae and T. castaneum for all exposure periods. No insects from the three species tested were able to chew through miniature ZeroFly bags, indicating the bag fabric will prevent entry or exit of insects.Peer reviewedEntomology and Plant Patholog

Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum

Background: Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide.Results: To understand the molecular bases of phosphine resistance in insects, we used RNA-Seq to compare gene expression in phosphine-resistant and susceptible laboratory populations of the red flour beetle, Tribolium castaneum. Each population was evaluated as either phosphine-exposed or no phosphine (untreated controls) in triplicate biological replicates (12 samples total). Pairwise analysis indicated there were eight genes differentially expressed between susceptible and resistant insects not exposed to phosphine (i.e., basal expression) or those exposed to phopshine (>8-fold expression and 90 % C.I.). However, 214 genes were differentially expressed among all four treatment groups at a statistically significant level (ANOVA, p < 0.05). Increased expression of 44 cytochrome P450 genes was found in resistant vs. susceptible insects, and phosphine exposure resulted in additional increases of 21 of these genes, five of which were significant among all treatment groups (p < 0.05). Expression of two genes encoding anti-diruetic peptide was 2- to 8-fold reduced in phosphine-resistant insects, and when exposed to phosphine, expression was further reduced 36- to 500-fold compared to susceptible. Phosphine-resistant insects also displayed differential expression of cuticle, carbohydrate, protease, transporter, and many mitochondrial genes, among others. Gene ontology terms associated with mitochondrial functions (oxidation biological processes, monooxygenase and catalytic molecular functions, and iron, heme, and tetrapyyrole binding) were enriched in the significantly differentially expressed dataset. Sequence polymorphism was found in transcripts encoding a known phosphine resistance gene, dihydrolipoamide dehydrogenase, in both susceptible and resistant insects. Phosphine-resistant adults also were resistant to knockdown by the pyrethroid deltamethrin, likely due to the increased cytochrome P450 expression.Conclusions: Overall, genes associated with the mitochondria were differentially expressed in resistant insects, and these differences may contribute to a reduction in overall metabolism and energy production and/or compensation in resistant insects. These data provide the first gene expression data on the response of phosphine-resistant and -susceptible insects to phosphine exposure, and demonstrate that RNA-Seq is a valuable tool to examine differences in insects that respond differentially to environmental stimuli.Peer reviewedEntomology and Plant Patholog

Insecticidal Effect of Diatomaceous Earth Against Three Species of Stored-Product Psocids on Maize, Rice, and Wheat

We evaluated the efficacy of three diatomaceous earth (DE) formulations, Dryacide, Protect-It, and Insecto, against three Psocoptera species, Liposcelis entomophila (Enderlein) (Liposcelididae), Lepinotus reticulatus Enderlein (Trogiidae), and Liposcelis decolor (Pearman), in the laboratory. Bioassays were conducted in three grain commodities, wheat, rice and maize, at 30°C and 75% RH, and the DEs were applied at the recommended dose rates of 1,000, 400, and 500 ppm for Dryacide, Protect-It, and Insecto, respectively. Differences in adult mortality were found among grains and DEs for L. entomophila and L. reticulatus, but these trends were not consistent for all combinations tested. Type of grain and DE did not affect L. decolor mortality significantly. Moreover, mortality increased with increasing exposure time for L. entomophila and L. reticulatus, but there was no effect of exposure time on L. decolor. After 7 d of exposure, mortalities of L. entomophila, L. reticulatus, and L. decolor were 56, 55, and 40%, respectively, and the respective mortality levels after 14 d were 63, 71, and 42%. Progeny production after 30 d was significantly suppressed for all species in the treated grains. However, progeny production was still high in the treated grains and reached 54, 42, and 76 individuals/10 g of grain for L. entomophila, L. reticulatus, and L. decolor, respectively. Progeny production did not vary with commodity. Our results suggest that DEs, when used alone, will not provide effective control of psocids